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In vivo expression of IL‐4, IL‐5, IL‐13 and IFN‐γ mRNAs in peripheral blood mononuclear cells and effect of cyclosporin A in a patient with Kimura's disease
Author(s) -
KATAGIRI K.,
ITAMI S.,
HATANO Y.,
YAMAGUCHI T.,
TAKAYASU S.
Publication year - 1997
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1997.19962077.x
Subject(s) - peripheral blood mononuclear cell , cytokine , immunology , interleukin 4 , messenger rna , immunoglobulin e , in vivo , interleukin 5 , interferon gamma , medicine , interleukin , biology , microbiology and biotechnology , in vitro , antibody , gene , biochemistry
Summary Semiquantitative reverse transcription‐polymerase chain reaction was used to analyse the expression of cytokine mRNAs in freshly isolated peripheral blood mononuclear cells obtained from a patient with Kimura's disease. The patient was treated with cyclosporin A (CsA) after incomplete tumour resection and irradiation of lesions. Levels of interleukin (IL)‐4, IL‐5 and IL‐13 mRNA were elevated and the level of interferon (IFN)‐γ mRNA was within normal limits before treatment. The levels of IL‐4, Il‐5 and IL‐13 mRNA, the number of eosinophils, and the serum level of IgE decreased markedly after surgery and radiation therapy. CsA treatment suppressed these values in a dose‐dependent manner, but had a minimal effect on the level of IFN‐γ mRNA. The number of peripheral eosinophils decreased in association with decreases in the levels of IL‐4, IL‐5 and IL‐13 mRNAs during CsA therapy; the serum level of IgE remained low during therapy and did not fluctuate in association with changes in cytokine levels. These results suggest the Th2 cytokines play a part in the development of Kimura's disease and that CsA suppresses the activity of this disease.