Premium
Blockade of costimulatory molecules B7‐1 (CD80) and B7‐2 (CD86) down‐regulates induction of contact sensitivity by haptenated epidermal cells
Author(s) -
KATAYAMA I.,
MATSUNAGA T.,
YOKOZEKI H.,
NISHIOKA K.
Publication year - 1997
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1997.01842.x
Subject(s) - hapten , cd86 , cd80 , chemistry , microbiology and biotechnology , antibody , immunology , in vitro , t cell , immune system , biology , biochemistry , cytotoxic t cell , cd40
Summary The hapten, trinitrobenzene sulphonic acid, induced weak B7‐1 (CD80) and moderate B7‐2 (CD86) expression on Langerhans cells and mRNA expression of both molecules in organ‐cultured murine skin. The intradermal injection of hapten‐treated epidermal cells induced hapten‐specific contact sensitivity in synergic mice. Cells of the keratinocyte cell line, Pam 212, or epidermal cells treated with a mixture of anti‐Ia/thy 1·2/γδ antibody plus complement, did not show any sensitizing ability. When hapten‐treated epidermal cells were injected into mice after incubation with anti‐B7‐2 (CD86) or B7‐1 (CD80) antibody the resultant contact sensitivity reaction was decreased to less than 50% of the control reaction, a reduction which was similar to that seen with the anti‐ICAM‐1 and anti‐LFA‐1 antibody‐induced inhibition of contact sensitivity. Anti‐B7‐1 (CD80) or anti‐B7‐2 (CD86) antibody also inhibited hapten‐specific lymphocyte proliferation or the allogenic mixed lymphocyte and epidermal cell reaction in vitro , although the inhibitory effect of anti‐B7‐1 antibody was not as significant as that of anti‐B7‐2 antibody. These results indicate that costimulatory signals induced by a hapten on epidermal Langerhans cells play an important role in the induction of hapten‐specific contact sensitivity in mice.