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Decreased β2‐adrenergic receptor‐mRNA and Ioricrin‐mRNA, and increased involucrin‐mRNA transcripts in psoriatic epidermis: analysis by reverse transcription–polymerase chain reaction
Author(s) -
TAKAHASHI H.,
KINOUCHI M.,
TAMURA T.,
IIZUKA H.
Publication year - 1996
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1046/j.1365-2133.1996.d01-903.x
Subject(s) - involucrin , loricrin , epidermis (zoology) , messenger rna , biology , microbiology and biotechnology , reverse transcription polymerase chain reaction , filaggrin , gene expression , real time polymerase chain reaction , keratinocyte , endocrinology , gene , immunology , biochemistry , in vitro , anatomy , atopic dermatitis
Summary Psoriatic hyperproliferative epidermis is characterized by a decreased β 2 ‐adrenergic adenylate cyclase response as well us by altered differentiation markers that include decreased loricrin and increased involucrin, Using a quantitative reverse transcription‐polymerase chain reaction, we analysed the expression of β 2 ‐adrenergic receptor‐mRNA. loricrin‐mRNA, and involucrin‐mRNA in the epidermis of five patients with psoriasis vulgaris. The mRNAs of the β 2 ‐adrenergic receptor and loricrin in the involved epidermis were significantly decreased, by 0.35‐fold ( P < 0.01) and 0.55‐fold ( P < 0.05) respectively, compared with uninvolved epidermis. In contrast, the involucrin mRNA expression of the involved epidermis was significantly increased, by 3.77‐fold ( P < 0.01). No significant difference in β ‐actin mRNA transcripts was detected between the involved and the uninvolved epidermis. These results indicate that the altered expression of the β 2 ‐adrenergic receptor, loricrin. and involucrin. in the psoriatic involved epidermis, is associated with different amounts of each mRNA transcripts.