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Genotyping of four genetic polymorphisms in the CYP1A2 gene in the Egyptian population
Author(s) -
Hamdy Samar I.,
Hiratsuka Masahiro,
Narahara Kaori,
Endo Naomi,
ElEnany Mervat,
Moursi Nadia,
Ahmed Mohammed S.E.,
Mizugaki Michinao
Publication year - 2003
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1046/j.1365-2125.2003.01787.x
Subject(s) - genotyping , allele frequency , cyp1a2 , genetics , population , biology , polymorphism (computer science) , allele , genotype , gene , medicine , environmental health , in vitro , microsome
AimsThe goal of this study was to determine the frequencies of CYP1A2 * 1C , * 1D , * 1E and * 1F variants in the Egyptian population and compare frequencies with other populations.MethodsGenotyping was performed in a total of 212 unrelated Egyptian subjects using polymerase chain reaction‐restriction fragment length polymorphism assay.ResultsThe frequencies of CYP1A2 * 1C , * 1D , * 1E and * 1F variants in the Egyptian population were 0.07, 0.40, 0.03 and 0.68, respectively. The Egyptians have a lower frequency of CYP1A2 * 1C , and CYP1A2 * 1E than the Japanese (0.07 vs 0.21 and 0.03 vs 0.08, respectively), while the frequencies of CYP1A2 * 1D and CYP1A2 * 1F did not differ significantly between the two groups. CYP1A2 * 1F (0.68) frequency in Egyptians was identical to that observed in Caucasians (0.68 among 236 German individuals).ConclusionsThe present study is the first to describe the frequencies of four known allelic variants of CYP1A2 among the Egyptian population. CYP1A2 * 1C and * 1E occurred at frequencies significantly lower than that in Japanese, while similar frequencies were observed for CYP1A2 * 1D and * 1F. The CYP1A2 * 1F frequency appeared to be identical to that of Caucasians. This does not exclude the possibility of the presence of new mutations relatively specific to the Egyptian population that have not been identified.