Premium
Validation of a new fluorogenic real‐time PCR assay for detection of CYP2C9 allelic variants and CYP2C9 allelic distribution in a German population
Author(s) -
Burian Maria,
Grösch Sabine,
Tegeder Irmgard,
Geisslinger Gerd
Publication year - 2002
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1046/j.1365-2125.2002.01693.x
Subject(s) - genotyping , cyp2c9 , allele , genotype , biology , concordance , melting curve analysis , genetics , high resolution melt , population , allele frequency , microbiology and biotechnology , real time polymerase chain reaction , medicine , gene , environmental health
Aims The aim was to develop a fast detection method for the polymorphic alleles related to impaired CYP2C9‐mediated metabolism. Methods CYP2C9 genotypes were identified in 118 DNA samples using real‐time PCR (LightCycler) followed by melting curve analysis. All samples were re‐tested by validated PCR‐RFLP methodology. Results The concordance between the two methods was 100% for two variant alleles. The frequencies of CYP2C9 * 1 (wild type), CYP2C9*2 and CYP2C9*3 (with 95% confidence intervals) were 0.81(0.05), 0.14(0.04) and 0.05(0.03), respectively, and are similar to those observed in other Caucasian populations. Conclusions This assay is simple and rapid and may be used for CYP2C9‐genotyping in a clinical setting.