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Effects of nociceptin and endomorphin 1 on the electrically stimulated human vas deferens
Author(s) -
Bigoni Raffaella,
Calo Girolamo,
Guerrini Remo,
Strupish James W.,
Rowbotham David J.,
Lambert David G.
Publication year - 2001
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1046/j.1365-2125.2001.01356.x
Subject(s) - vas deferens , nociceptin receptor , (+) naloxone , antagonist , stimulation , chemistry , endocrinology , receptor , medicine , pi , opioid receptor , morphine , pharmacology , opioid , opioid peptide , biochemistry
Aims To examine the effects of nociceptin (NC) and endomorphin 1 (EM1) on electrical field stimulation (EFS)‐induced contractions of the human vas deferens (hVD). Methods Concentration‐response curves to NC and EM1 were constructed in the absence and in presence of peptidase inhibitors (PI). In some experiments a NC receptor antagonist, [Phe 1 ψ(CH 2 ‐NH)Gly 2 ]NC(1–13)NH 2 [F/G], 10 µ m ) or naloxone (1 µ m ) were included. Results All data are mean(95%CI). In the presence of PI, NC inhibited twitches (E max =67(44,90)%; pE C 50 = 7.28(6.95,7.61)). NC inhibition was sensitive to [F/G]. EM1 also inhibited twitches both in the absence (E max =82(73,91)% pE C 50 = 7.07(6.92,7.22)) and presence (E max =83(76,90)%; pE C 50 = 7.00(6.91, 7.09)) of PI. EM1 inhibition was sensitive to naloxone. Conclusions These data suggest that hVD express NC and opioid receptors that inhibit neurogenic contractions.