Premium
Carbon and nitrogen dynamics in zero‐water exchange shrimp culture as indicated by stable isotope tracers
Author(s) -
Epp M A,
Ziemann D A,
Schell D M
Publication year - 2002
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1046/j.1365-2109.2002.00720.x
Subject(s) - shrimp , biology , nitrogen , glycine , stable isotope ratio , environmental chemistry , isotopes of carbon , isotopes of nitrogen , aquaculture , δ13c , carbon fibers , tracer , amino acid , ecology , biochemistry , fishery , chemistry , total organic carbon , fish <actinopterygii> , physics , materials science , quantum mechanics , composite number , composite material , organic chemistry , nuclear physics
The uptake and assimilation of nitrogen and carbon by shrimp were measured in 1200 L mesocosms using stable isotope enrichments. Labels were added via 15 N‐, 13 C‐glycine and amino acid mixtures in feeds or as 15 NH 4 + to pond water. Label was incorporated into shrimp via algal growth indicating that up to 31% of nitrogen requirements were derived from pond ecosystem dynamics. This value is low in comparison with other shrimp aquaculture isotopic tracer studies but is probably due to differences in shrimp‐rearing conditions. Direct incorporation of the enriched feed label was low in shrimp muscle tissue (3.3% for 13 C‐glycine, 5.9% for 15 N‐glycine and 7.8% for 15 N‐amino acid mixture). Mass balance calculations indicate the remaining shrimp biomass was derived from feed, but loss of label into solution during feeding led to underestimation based on tracers. Incorporation of isotopic labels into feed as large molecular weight proteinaceous or microencapsulated/fat‐coated compounds is recommended to prevent dissolution and loss.