Premium
Effect of induced hyperoxia on the antioxidant status of Atlantic salmon Salmo salar L. fed three different levels of dietary vitamin E
Author(s) -
Lygren B.,
Hamre K.,
Waagbø R.
Publication year - 2000
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1046/j.1365-2109.2000.00459.x
Subject(s) - salmo , biology , hyperoxia , vitamin e , antioxidant , vitamin , zoology , antioxidant capacity , fishery , fish <actinopterygii> , biochemistry , medicine , lung
Atlantic salmon smolt (64 g) were fed 40, 300 or 1100 mg kg −1 all‐rac‐α‐tocopheryl acetate feed under normoxic and moderate hyperoxic conditions for 12 weeks. After 7 weeks, however, the hyperoxic fish were subjected to a temporary severe supersaturation of dissolved oxygen with development of gas bubble disease and substantial mortality as a result. Liver α‐tocopherol (α‐TOH) levels reflected the dietary input after 6 and 12 weeks of feeding. Increased levels of thiobarbituric‐reactive substances (TBARS) in the liver of hyperoxygenated fish after 6 weeks indicate that moderate hyperoxic conditions may represent an oxidative challenge to the fish. After 12 weeks, liver ascorbic acid (AA) content was reduced in hyperoxygenated fish, and a decreasing tendency was also found in the liver content of α‐TOH. Effects of oxygen supersaturation were also observed on liver antioxidant enzyme activities, content of glutathione and total mercapthans in liver, phagocytic chemiluminescent response and total plasma protein level, indicating a disturbed redox balance. No effects of dietary vitamin E were observed on any of the measured parameters, indicating that high levels of dietary vitamin E under these circumstances did not protect the fish against oxidative stress.