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Computer‐controlled freezing of rainbow trout Oncorhynchus mykiss (Walbaum) spermatozoa for routine programmes
Author(s) -
Babiak I.,
Fraser L.,
Dobosz S.,
Goryczko K.,
Kuzminski H.,
Strzezek J.
Publication year - 1999
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1046/j.1365-2109.1999.00381.x
Subject(s) - rainbow trout , milt , extender , biology , cryopreservation , human fertilization , oncorhynchus , sperm , zoology , fishery , andrology , fish <actinopterygii> , anatomy , embryo , botany , chemistry , medicine , organic chemistry , polyurethane
The effects of extender composition and freezing rate on cryopreservation efficiency of refrigerated spermatozoa of rainbow trout Oncorhynchus mykiss (Walbaum) were evaluated in order to test the suitability of a computer‐controlled ultrafreezer to cryopreserve milt samples obtained in field conditions and stored for several hours. A very highly significant first‐order interaction between freezing rate and the type of extender was found. Six of the eight experimental variants did not differ significantly, resulting, after fertilization of eggs with cryopreserved sperm, in a range of 62.3–74.8% of eyed embryos. This procedure was effective for samples stored at 1 °C for 2 days.