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The effect of individual male potency on fertilization ability of fresh and cryopreserved milt of rainbow trout, Oncorhynchus mykiss (Walbaum)
Author(s) -
Babiak I,
Glogowski J,
Luczynski M,
Goryczko K,
Dobosz S,
Kuzminski H
Publication year - 1998
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1046/j.1365-2109.1998.00197.x
Subject(s) - milt , rainbow trout , biology , human fertilization , fishery , cryopreservation , potency , zoology , anatomy , fish <actinopterygii> , biochemistry , embryo , in vitro
Abstract The goal of the present study was to examine individual male potency in rainbow trout, Oncorhynchus mykiss (Walbaum), expressed as the fertilization ability of fresh and cryopreserved sperm. One female and four males bearing genetic markers enabling determination of the progeny paternity were chosen as gamete donors. Samples of eggs were inseminated with sperm from separate individuals or with pooled sperm. Genetic examination of the progeny obtained after fertilization of eggs with pooled milt showed differences in male potency. The proportions of offspring sired by four individual males after fertilization of eggs with the fresh milt were similar to those obtained after fertilization with cryopreserved milt (correlation r = 0.95; n = 4; P < 0.05). These proportions did not correlate with the proportions of progeny resulting from fertilization of eggs when sperm was not pooled.