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DNA fingerprinting in Indian major carps and tilapia by Bkm 2(8) and M13 probes
Author(s) -
Majumdar K C,
Ravinder K,
Nasaruddin K
Publication year - 1997
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1046/j.1365-2109.1997.t01-1-00837.x
Subject(s) - biology , labeo , catla , oreochromis mossambicus , genetics , dna profiling , population , inbreeding , taqi , zoology , dna , allele , gene , tilapia , fishery , fish <actinopterygii> , polymorphism (computer science) , demography , sociology
DNA fingerprints were obtained in three species of commercially important freshwater fishes, Labeo rohita (Hamilton). Catla catla (Hamilton) and Oreachromis mossambicus (Peters), using Bkm 2(8) and M13 multilocus probes. Bkm 2(8) gave a higher number of bands when compared with M13. However, the number of bands obtained by each probe in O. mossambicus was similar. The higher band‐sharing coefficient observed in this species may be attributed to inbreeding as it arose from a small founder population. In L rohita and C. catla , the Bkm 2(8) detected similar DNA fingerprints when two enzymes Hinfi and Taqi were used. The M13 probe also gave similar fingerprints with three restriction enzymes (Hinfi, Taqi, Alui). Comparison of the DNA fingerprints obtained by Bkm 2(8) and Ml 3 showed that these two probes detected different alleles. The overall similarity of the DNA fingerprint patterns in L. rohita and C. catla may be due to their genetic closeness as indicated by their same chromosome number, C ‐value and their ability to produce fertile hybrids. A similar argument also holds true for the Oreochromis species where interspecies hybridization results in fertile offspring.