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Cryopreservation of sperm of common carp, Cyprinus carpio L.
Author(s) -
Babiak I,
Glogowski J,
Brzuska E,
Szumiec J,
Adamek J
Publication year - 1997
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1046/j.1365-2109.1997.00897.x
Subject(s) - cyprinus , extender , milt , biology , common carp , yolk , sperm , cryopreservation , zoology , carp , larva , anatomy , fishery , andrology , fish <actinopterygii> , embryo , botany , chemistry , medicine , organic chemistry , polyurethane
Milt of common carp, Cyprinus carpio L. was cryopreserved in pellet form with the use of 12 extenders. Most efficient were: BE2 original extender (containing 85 mM NaCl, 50 mM KCl, 3 m m CaCl 2 , 1 m m MgCl 2 with 10% dimethyl‐acetamide (DMA) and 10% addition of hen's egg yolk) and Kurokura et al. 's extender with 15% DMA and 10% yolk (about 73% and 69% of eyed eggs, about 61% and 52% of swim‐up larvae, respectively). Within the most effective treatments, survival from the eyed‐egg stage to the swim‐up stage was similar to that observed in the control group. Survival from the eyed‐egg stage to the swim‐up stage (percentage of eyed eggs was considered as 100%) was highly significantly and positively correlated with the actual rate of swim‐up larvae.