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Identification of a sex‐determining region in Nile tilapia ( Oreochromis niloticus ) using bulked segregant analysis
Author(s) -
Lee B.Y.,
Penman D. J.,
Kocher T. D.
Publication year - 2003
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2003.01035.x
Subject(s) - biology , bulked segregant analysis , heterogametic sex , genetics , locus (genetics) , microsatellite , nile tilapia , sex linkage , w chromosome , genetic marker , allele , genetic linkage , broodstock , population , centimorgan , linkage disequilibrium , oreochromis , haplotype , gene mapping , chromosome , gene , fishery , karyotype , aquaculture , demography , sociology , fish <actinopterygii>
Summary Sex determination in the Nile tilapia ( Oreochromis niloticus ) is thought to be an XX‐XY (male heterogametic) system controlled by a major gene. We searched for DNA markers linked to this major locus using bulked segregant analysis. Ten microsatellite markers belonging to linkage group 8 were found to be linked to phenotypic sex. The putative Y‐chromosome alleles correctly predict the sex of 95% of male and female individuals in two families. Our results suggest a major sex‐determining locus within a few centimorgans of markers UNH995 and UNH104 . A third family from the same population showed no evidence for linkage of this region with phenotypic sex, indicating that additional genetic and/or environmental factors regulate sex determination in some families. These markers have immediate utility for studying the strength of different Y chromosome alleles, and for identifying broodstock carrying one or more copies of the Y haplotype.