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Mutations in the limbin gene previously associated with dwarfism in Japanese brown cattle are not responsible for dwarfism in the American Angus breed
Author(s) -
Mishra B. P.,
Reecy J. M.
Publication year - 2003
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2003.01020.x
Subject(s) - dwarfism , biology , breed , genetics , library science , gene , computer science
Source/description of primers: A mutation in the ovine bonemorphogenetic protein receptor-1B (BMPR1B) gene is associated with an increased ovulation rate phenotype in Booroola Merino sheep. Therefore, BMPR1B is considered as a candidate gene for ovulation rate in cattle. A cDNA was obtained for porcine BMPR1B and the gene was mapped in the porcine genome. Comparison of the ovine and porcine BMPR1B cDNA sequences indicated that they are highly conserved. Therefore, a pair of primers derived from the porcine BMPR1B cDNA, which amplified across intron 8 in the pig, were used to amplify the bovine genomic DNA. The forward (NZ-F1) and reverse (exon9-R1) primers correspond to bases 1041–1064 and 1289–1265 of the porcine BMPR1B cDNA (GenBank accession no. AF432128), respectively. Agarose gel electrophoresis and sequencing of the polymerase chain reaction (PCR) amplicons of bovine genomic DNA indicated that the size of this product was 1253 bp (GenBank accession no. AY242067).