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Characterization and mapping of bovine dopamine receptors 1 and 5
Author(s) -
Haegeman A.,
Williams J. L.,
Law A.,
Van Zeveren A.,
Peelman L. J.
Publication year - 2003
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2003.00994.x
Subject(s) - biology , complementary dna , genetics , coding region , allele , allele frequency , polymerase chain reaction , microbiology and biotechnology , snp , single nucleotide polymorphism , gene , genotype
Summary A cDNA encoding the bovine dopamine receptor 1 ( DRD1 ) was isolated from a bovine cDNA library, cloned and completely sequenced. The coding region showed 93 and 91% sequence identity on DNA level and 96 and 94% on protein level with its respective porcine and human orthologs. The bovine DRD1 and dopamine receptor 5 ( DRD5 ) were mapped, respectively, to BTA10 and 6 by radiation hybrid mapping. One SNP was found in DRD1 and four in DRD5. Using polymerase chain reaction–restriction fragment length polymorphism, 11 different European cattle breeds were screened for the presence of the DRD1 and DRD5 substitutions. Allele frequencies for DRD1 and DRD5 alleles were very similar across all the breeds examined. Allele frequency discrepancies were found between Belgian Blue beef breed and the other breeds.