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Genetic diversity of three donkey populations in the Croatian coastal region
Author(s) -
Ivankovic A.,
Kavar T.,
Caput P.,
Mioc B.,
Pavic V.,
Dovc P.
Publication year - 2002
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2002.00879.x
Subject(s) - biology , haplotype , donkey , microsatellite , population , genetic diversity , croatian , genetic variation , genetics , range (aeronautics) , d loop , analysis of molecular variance , mitochondrial dna , allele , ecology , gene , demography , linguistics , philosophy , materials science , sociology , composite material
Genetic variation in three Croatian donkey populations, Istrian (IS), North Adriatic (NA) and Littoral‐Dinaric (LD), was analysed using eight microsatellite loci and by sequence and SSCP analysis of the proximal portion of the mtDNA D‐loop region. The analysis of microsatellite loci revealed observed heterozygosities in the range of 0.37 ( MPZ002 in LD) to 0.85 ( AHT21 in LD) and polymorphic information content values in the range of 0.36 ( MPZ002 in NA) to 0.78 ( AHT21 in LD). The overall probability of exclusion was 0.991. Two populations (IS and NA) were closely related (Fst=0.0034), whereas genetic distances between IS and LD (Fst=0.021) and NA and LD (Fst=0.027) were higher. Using AMOVA , 97.6% of the total genetic variance was portioned within populations, while 2.7% was portioned between the Littoral‐Dinaric population and the Istrian/North Adriatic population group. Sequencing of the proximal part of the mtDNA D‐loop region revealed 36 polymorphic sites representing 19 haplotypes which clustered into three haplotype groups (Y, W, Ws). Only the Y haplotype was found in the IS population which is characterized by a large body size. Haplotypes W and Ws were found in the NA and LD populations which include smaller animals. All three haplotypes were found in the LD population, indicating sporadic migration events from the IS into LD donkey population.