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MHC‐DRB exon 2 allele polymorphism in Indian river buffalo ( Bubalus bubalis )
Author(s) -
De S.,
Singh R. K.,
Butchaiah G.
Publication year - 2002
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2002.00838.x
Subject(s) - bubalus , exon , biology , genetics , single strand conformation polymorphism , locus (genetics) , allele , major histocompatibility complex , heteroduplex , microbiology and biotechnology , gene , ecology
The polymorphism of the major histocompatibility complex (MHC) class II DRB gene of riverine buffalo ( Bubalus bubalis ) was studied. Second exon sequences from the buffalo DRB locus, homologous to the cattle DRB3 gene, were amplified and characterized. A combination of single strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) in a non‐denaturing gel was used to identify new DRB second exon sequences. SSCP, HA and finally sequencing allowed the identification of 22 MHC‐DRB exon 2 alleles from 25 unrelated Indian river buffalo. These are the first river buffalo DRB second exon sequences reported. A high degree of polymorphism in the sequences encoding the peptide binding regions was observed and some amino acid substitutions were found unique to the river buffalo.