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A set of cattle microsatellite DNA markers for genome analysis of riverine buffalo ( Bubalus bubalis )
Author(s) -
Navani N.,
Jain P. K.,
Gupta S.,
Sisodia B. S.,
Kumar S.
Publication year - 2002
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2002.00823.x
Subject(s) - biology , microsatellite , bubalus , loss of heterozygosity , genotyping , primer (cosmetics) , genetics , genome , genetic marker , allele , amplicon , genotype , polymerase chain reaction , gene , organic chemistry , ecology , chemistry
One hundred and eight microsatellite primer pairs, originally identified from cattle, were evaluated for their applicability in buffalo. Eighty‐one primer pairs (75%) amplified discrete products, and of these, 61 pairs (56%) gave polymorphic band patterns on a panel of 25 buffaloes. The mean number of alleles per polymorphic marker was 4.50 ± 0.20, and the mean heterozygosity per polymorphic marker was 0.66 ± 0.02. Successful genotyping of buffaloes using cattle specific primers suggests that the latter can be a valuable resource for genome analysis in bubaline species.