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Mapping of 12 bovine ribosomal protein genes using a bovine radiation hybrid panel
Author(s) -
Wang Y.H.,
McWilliam S. M.,
Barendse W.,
Kata S. R.,
Womack J. E.,
Moore S. S.,
Lehnert S. A.
Publication year - 2001
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2001.00791.x
Subject(s) - biology , ribosomal protein , intron , genetics , gene , ribosomal rna , ribosomal dna , exon , 28s ribosomal rna , complementary dna , microbiology and biotechnology , ribosome , phylogenetic tree , rna
Twelve bovine ribosomal protein genes, for which sequence data had been acquired from complementary deoxyribonucleic acid (cDNA) clones isolated from a cattle skin cDNA library, were mapped. As ribosomal protein genes are a group of highly conserved house keeping genes, specific primers were designed to span the intron–exon splice sites and to amplify intronic sequences, in order to obtain bovine‐specific polymerase chain reaction (PCR) products. Two of 12 ribosomal protein genes were genotyped in this way and the remaining 10 were mapped using additional primers designed from within the intron. Eleven previously unmapped ribosomal protein genes were localized and one previously reported ribosomal protein gene localization was confirmed. The 12 ribosomal protein genes mapped in this study are spread over 10 chromosomes, including the X chromosome. The locations show conservation of comparative map position in cattle and human.