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Isolation of expressed sequence tags of skeletal muscle of neonatal healthy and splay leg piglets and mapping by somatic cell hybrid analysis
Author(s) -
Maak S.,
Jaesert S.,
Neumann K.,
Yerle M.,
Von Lengerken G.
Publication year - 2001
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2001.00778.x
Subject(s) - biology , expressed sequence tag , genbank , gene , genetics , skeletal muscle , complementary dna , ribosomal protein , microbiology and biotechnology , anatomy , ribosome , rna
We have isolated 14 differentially displayed and 10 further expressed sequence tags (ESTs) from Musculus biceps femoris of newborn healthy and splay leg piglets. By comparison with EMBL/GenBank data we could identify nine porcine homologues to human genes (TATA box binding protein associated factor B TAF1B; B‐cell CLL/lymphoma 7B BCL7B; pyruvate dehydrogenase kinase, isoenzyme 4 PDK4; ribosomal protein S10 RPS10; SPARC‐like 1 SPARCL1; epithelial protein lost in neoplasm β EPLIN; N‐myc downstream‐regulated gene 2 NDRG2; pleiomorphic adenoma gene like 2 PLAGL and, BCL‐2 associated transcription factor short form BTFS ). Eight fragments correspond to uncharacterized ESTs and 7 ESTs had no significant match with database sequences. These data provide the first expression profiles in skeletal muscle of neonatal piglets and are a basis for candidate gene investigations for congenital splay leg in piglets. Eleven ESTs were physically mapped to porcine chromosomes 1, 3, 4, 5, 7, 8, 9 and 10 and contribute to the comparative map of humans and pigs.