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Transcriptome of channel catfish ( Ictalurus punctatus ): initial analysis of genes and expression profiles of the head kidney
Author(s) -
Cao D.,
Kocabas A.,
Ju Z.,
Karsi A.,
Li P.,
Patterson A.,
Liu Z.
Publication year - 2001
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2001.00753.x
Subject(s) - ictalurus , biology , catfish , transcriptome , expressed sequence tag , gene , genetics , gene expression profiling , complementary dna , cdna library , ictaluridae , functional genomics , gene expression , computational biology , genome , genomics , fish <actinopterygii> , fishery
Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish ( Ictalurus punctatus ), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.