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Development and mapping of microsatellites from a microdissected BTA 11‐specific DNA library
Author(s) -
Ambady S.,
Kappes S. M.,
Park C.,
Ma R. Z.,
Beever J. E.,
Lewin H. A.,
Smith T. P. L.,
Beattie C. W.,
Basrur P. K.,
De León F. Abel Ponce
Publication year - 2001
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2001.00744.x
Subject(s) - biology , autosome , microsatellite , genetics , chromosome , microdissection , chromosomal translocation , genomic library , bovine genome , microbiology and biotechnology , chromosome 17 (human) , chromosome 22 , dna , gene , genome , allele , base sequence
A chromosome‐specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC) 12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11‐specific microsatellite markers. This study suggests that chromosome‐specific libraries have great potential for development of microsatellite markers for the construction of marker‐saturated linkage maps for each chromosome.