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Mapping of 14 expressed sequence tags (ESTs) from porcine skeletal muscle by somatic cell hybrid analysis
Author(s) -
Davoli R,
Bigi D,
Fontanesi L,
Zambonelli P,
Yerle M,
Zijlstra C,
Bosma A A,
Robic A,
Russo V
Publication year - 2000
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2000.00687.x
Subject(s) - expressed sequence tag , biology , cdna library , genetics , complementary dna , human genome , radiation hybrid mapping , skeletal muscle , sequence tagged site , genome , computational biology , gene , gene mapping , chromosome , anatomy
Chromosomal assignments are reported for fourteen porcine expressed sequence tags (ESTs) — CALM1 , CRYAB , MYH7 , MYL1 , PDK4 , PGAM2 , PYGM , REV3L , RFC1 , SLN , SPTBN1 , SRM160 , TPM1 and YWHAG . The ESTs were derived from our porcine skeletal muscle cDNA library. The ESTs sequences selected for mapping included the presence of the 3′‐untranslated region. The assignments were performed using two independent somatic cell hybrid panels providing the possibility of confirmation of the results obtained. The observed localizations are compared with the locations predicted from heterologous (human–pig, pig–human) chromosome painting data and knowledge of the map locations of the human homologues. These results add new information to the porcine genome transcript map.