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Construction and characterization of an ovine BAC contig spanning the callipyge locus
Author(s) -
Segers K,
Berghmans S,
Georges M,
Charlier C,
Vaiman D,
Shay T,
Cockett N,
Meyers S,
Beever J
Publication year - 2000
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2000.00676.x
Subject(s) - contig , locus (genetics) , biology , centimorgan , genetics , bacterial artificial chromosome , gene mapping , microbiology and biotechnology , chromosome , gene , genome
We describe the construction of an ovine BAC contig spanning a 4·6 centimorgan (cM) chromosome segment known to contain the callipyge ( CLPG ) locus. The contig comprises 21 ovine BAC clones jointly covering approximately 900 kilobases (Kb). Two gaps in the BAC contig, spanning 10 and 7·5 Kb, respectively, were bridged by long range PCR. The corresponding chromosome region was shown to be characterized by an unusually low Kb to cM ratio (164 Kb/cM) and a high density of Not I sites (1:126 Kb) possibly reflecting a high gene density in the corresponding chromosome region. Equivalent amplification of 64 sequence tagged sites spanning the corresponding region from homozygous +/+ and CLPG/CLPG individuals disproves the hypothesis of a major deletion causing the CLPG mutation.