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Biochemical, molecular and physiological characterization of a new β‐casein variant detected in Korean Cattle
Author(s) -
Han S K,
Shin Y C,
Byun H D
Publication year - 2000
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2000.00582.x
Subject(s) - biology , casein , polymorphism (computer science) , allele , microbiology and biotechnology , genetics , beta lactoglobulin , biochemistry , gene , whey protein
Summary There are seven known genetic variants of bovine β‐casein (β‐CN) ‐ A1, A2, A3, B, C, D and E. In this study, we identified a new genetic variant (named β‐CN H) which migrates slower than the other variants in acidic starch gel electrophoresis. We confirmed through protein and DNA sequence analyses that the H variant differs at five residues from the A2 sequence: Arg25/Cys, Leu88/Ile, Gln117/Glu, Glu175/Gln and Gln195/Glu. Of these substitutions the 25th residue was contained in the casein phosphopeptide (CPP) region. In rats, calcium solubilizing effect of the CPP of bovine variant H was increased by ≈ 23% compared with that of the CPP of non‐H. Using extensive Korean Bos taurus pedigrees, we confirmed that β‐CN H was controlled by a codominant allele.