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Identification and characterization of a new allele for the beta subunit of follicle‐stimulating hormone in Chinese pig breeds *
Author(s) -
Li M D,
Rohrer G A,
Wise T H,
Ford J J
Publication year - 2000
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2000.00581.x
Subject(s) - biology , allele , microbiology and biotechnology , single nucleotide polymorphism , genetics , untranslated region , allele frequency , genotype , gene , messenger rna
Summary During evaluation of follicle‐stimulating hormone‐beta ( FSHB ) expression in anterior pituitary glands by an RNase protection assay (RPA), the expected fragment of 205 nucleotides at positions 759–963 was not detected in one boar that had moderate plasma and pituitary FSH concentrations. After subcloning and sequencing, mRNA from this boar lacked an 11‐bp fragment (5′‐CATTTGGAAAC‐3′) at nucleotide positions 807–817 of the 3′‐untranslated region (3′‐UTR, D allele). Wild‐type FSHB ( WT allele) was present in pituitary RNA and genomic DNA in both Meishan (MS) and White Composite (WC) pigs; whereas the D allele was present only in MS pigs ( P < 0·01; 5/6 MS vs. 0/6 WC). Also, we found the D allele in five other Chinese breeds but absent in ten American Landrace, 11 Yorkshire and 17 Berkshire pigs. Additionally, the D allele had one silent nucleotide change in the coding region plus six, single nucleotide changes in the 3′‐UTR.