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Multicolour fluorescent detection and mapping of AFLP markers in chicken ( Gallus domesticus )
Author(s) -
Herbergs J.,
Siwek M.,
Crooijmans R. P. M. A.,
Van der Poel J. J.,
Groenen M. A. M.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00494.x
Subject(s) - amplified fragment length polymorphism , biology , taqi , ecori , genotyping , genetics , microsatellite , genetic linkage , restriction enzyme , primer (cosmetics) , genetic marker , restriction fragment length polymorphism , population , microbiology and biotechnology , genotype , dna , gene , allele , genetic diversity , chemistry , demography , organic chemistry , sociology
We describe the mapping of amplified restriction fragment polymorphism (AFLP™) markers in chicken ( Gallus domesticus ) using a multicolour fluorescent detection system. DNA was used from a population consisting of four families with a total of 183 F2 individuals. The enzyme combination Eco RI/ Taq I was used for double digestion, and fluorescently labelled fragments were analysed on an ABI PRISM 377 DNA sequencer. Polymorphic signals in the range of 50–500 bp were genotyped with the ABI PRISM™ Genotyper 2·0 software, which enabled the analysis of both dominant and incomplete dominant markers (with respect to AFLP, often referred to as codominant). In 19 sets consisting of 3 Eco RI/ Taq I primer pair combinations each, a total of 475 polymorphic markers was detected. From these polymorphisms 344 markers could be mapped on the Wageningen linkage map. Fourteen markers were length polymorphisms of the same fragment and 28 markers Z‐linked and uniformative; 64 AFLP markers appeared to be unlinked and 25 AFLP markers could not be accurately mapped on the basis of the genotyping results. The resulting AFLP/microsatellite linkage map is comprised of 33 linkage groups with a total of 835 loci.