Premium
The isolation and mapping of 19 tetranucleotide microsatellite markers in the chicken
Author(s) -
McConnell S. K. J.,
Dawson D. A.,
Wardle A.,
Burke T.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00454.x
Subject(s) - microsatellite , biology , genetics , genome , dinucleotide repeat , computational biology , gene , allele
Microsatellites consisting of tetranucleotide repeats are more easily, and consequently efficiently, scored than loci consisting ofdinucleotides. However, they are much less fre‐quent in the genome. A hybridisation selection protocol was therefore employed to generate a chicken genomic library enriched for inserts containing the tetranucleotide repeat motif (TTTC) n . Forty‐five new microsatellite sequences were isolated that mainly consisted of perfect repeats of the tetranucleotide (TTTC) motif. Nineteen markers were mapped in one or both of the East Lansing and Compton international chicken reference populations.