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Applicability of bovine microsatellite markers for population genetic studies on African buffalo ( Syncerus caffer )
Author(s) -
Van Hooft W. F.,
Hanotte O.,
Wenink P. W.,
Groen A. F.,
Sugimoto Y.,
Prins H. H. T.,
Teale A.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00453.x
Subject(s) - microsatellite , biology , loss of heterozygosity , genetic marker , allele , genetics , population , polymorphism (computer science) , allele frequency , gene , demography , sociology
The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5·0 ( SE = 0·2) and the mean heterozygosity per marker was 0·61 ( SE = 0·03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo.