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A convenient and efficient microsatellite‐based assay for resolving parentages in dogs
Author(s) -
Koskinen M. T.,
Bredbacka P.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00438.x
Subject(s) - microsatellite , biology , repeatability , multiplex , multiplex polymerase chain reaction , genetics , microbiology and biotechnology , computational biology , polymerase chain reaction , chromatography , gene , allele , chemistry
We present a practical and efficient parentage analysis test for dogs. The method makes use of ten polymorphic microsatellite markers combined in three multiplex‐PCR reactions. All three multiplexes can be performed under the same cycling conditions and using a multiple‐dye detection system the PCR‐products can be pooled for electrophoresis. Amplifications in four different thermal cyclers produced easily interpretable results throughout demonstrating the repeatability of the assay. In order to evaluate the method’s efficiency a total of 100 dogs from four different breeds were typed. Assuming one known parent, exclusion probabilities ranging from 99·34% to 99·93% were attained.