Premium
Mapping of the Na + , K + ‐ATPase subunit α 2 ( ATP1A2 ) and muscle phosphofructokinase ( PFKM ) genes in pig by somatic cell hybrid analysis
Author(s) -
Fontanesi L.,
Davoli R.,
Zijlstra C.,
Bosma A. A.,
Russo V.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00407.x
Subject(s) - biology , microbiology and biotechnology , complementary dna , gene , homology (biology) , somatic cell , synteny , genetics , chromosome
Two expressed sequence tags were isolated from a porcine skeletal muscle cDNA library and identified as the putative partial cDNAs of the porcine Na + , K + ‐ATPase subunit α 2 ( ATP1A2 ) and muscle phosphofructokinase ( PFKM ) genes after sequencing and homology search. Results of analysis of a pig–rodent somatic cell hybrid panel by PCR allowed the assignments of ATP1A2 to porcine chromosome (chr) 4 and of PFKM to porcine chr 5. These assignments support previously observed conservation of syntenic relationships between human chr 1 and porcine chr 4 and between human chr 12 and porcine chr 5.