Premium
Molecular structure of canine LINE‐1 elements in canine transmissible venereal tumor
Author(s) -
Choi Y.,
Ishiguro N.,
Shinagawa M.,
Kim CJ.,
Okamoto Y.,
Minami S.,
Ogihara K.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00400.x
Subject(s) - biology , retroposon , insert (composites) , open reading frame , genetics , homologous chromosome , line (geometry) , gene , sequence (biology) , direct repeat , microbiology and biotechnology , transposable element , peptide sequence , genome , mechanical engineering , geometry , mathematics , engineering
We determined the 4251‐bp sequence of open reading frame 2 (ORF2) of canine LINE‐1 retroposon that encodes 1275 amino acids. The truncated LINE‐1 inserts associated with transmissible venereal tumor (TVT) of dogs contained the 1378‐bp LINE‐1 insert (TVT‐LINE) flanked by 10‐bp direct repeats upstream to c‐myc gene. The TVT‐LINE elements were composed of 416 bp inverse sequences homologous to the complementary strand of the LINE‐1, a 5‐bp deletion and 962‐bp sequences homologous to the 3′ region of the LINE‐1.