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Eight microsatellite markers for South American camelids
Author(s) -
Penedo M. C. T.,
Caetano A. R.,
Cordova K.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00382-8.x
Subject(s) - microsatellite , biology , genbank , genetics , primer (cosmetics) , genomic dna , genomic library , insert (composites) , locus (genetics) , microbiology and biotechnology , dna , gene , base sequence , allele , mechanical engineering , chemistry , organic chemistry , engineering
Description: A small insert genomic library was prepared withllama ( Lama glama ) DNA cloned into pJCP1 vector, based on procedures described by Ostrander et al . 1. Construction of library and screening of clones for CA/TG repeats was carried out by Research Genetics (Huntsville, AL). Plasmids from positive clones were sequenced on ABI 377 automated DNA sequencers (Applied Biosystem, Foster City, CA) at the Veterinary Genetics Laboratory. Primer sequences and characteristics of microsatellites are presented in Table 1. 1 Characteristics of 8 microsatellites for South American camelidsLocus Repeat motif sClone
product
ize (Alleles
n )
rSize
ange
(bp) Genbank
accession
number Primer sequences (5′‐3′)LCA56(CA) 12 C(CA) 2 135 16 133–169 AF091122 ATGGTGTTTACAGGGCGTTG
GCATTACTGAAAAGCCCAGGLCA63(GT) 8 (GC) 4 AC(GT) 8 218 14 190–254 AF091123 TTACCCAGTCCTTCGTGGG
GGAACCTCGTGGTTATGGAALCA65(TG) 13 169 14 165–191 AF091124 CCCCTGTGGTTGAAT
AACTCAGCTGTTGTCAGGGGLCA66(CA) 13 223 24 220–262 AF091125 GTGCAGCGTCCAAATAGTCA
CCAGCATCGTCCAGTATTCALCA68(GT) 13 194 10 191–209 AF091126 TCCTGTCTGTGAGAAGGCTG
CCGAAGGTAAAATGGAALCA70(TA) 4 (CA) 11 (TA) 2 212 7 211–223 AF091127 TTCTGATGTATGGCATAGCGA
TTAAGAGCAGGATAALCA71(TA) 2 (CA) 9 137 6 136–150 AF091128 CAGACATATACCTGTATCCGTATCTA
TTCAGTGTTTCCTCGCAATGLCA77(GT) 3 A(TG) 8 235 15 233–263 AF091129 TGTTGACTAGAGCCTTTTCTTCTTT
GGGCAAGAGAGACTGACTGG