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The porcine Poly(rC)‐Binding Protein 2 ( PCBP2 ) gene maps to chromosome 5
Author(s) -
Davoli R.,
Fontanesi L.,
Č S.,
Musilová P.,
Stratil A.,
Rubeì J.,
Russo V.
Publication year - 1999
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1999.00382-7.x
Subject(s) - genbank , biology , complementary dna , microbiology and biotechnology , accession number (library science) , clone (java method) , recombinant dna , gene , alternative splicing , peptide sequence , insert (composites) , amino acid , rna splicing , genetics , protein sequencing , exon , mechanical engineering , rna , engineering
Description : A recombinant clone was isolated at random from a skeletal muscle cDNA library 1. The sequence of the resulting 598 bp insert (EMBL accession number X94253 ) was compared in EMBL and GenBank databases using BLASTN 2. The comparisons showed the highest matches with mouse and human PCBP2 cDNAs 3−5 (mouse X75947 , mouse X97982 , human X78136 and mouse L19661 ; BLASTN probability were 1·9e‐182, 1·1e‐176, 1·6e‐174 and 4·7 e‐172, respectively). Excluding two gaps that could be due to alternative splicing, the deduced porcine protein sequence revealed the complete identity with corresponding human ( X78136 ) and mouse ( L19661 ) sequences. The mouse sequences X75947 and X97982 showed only one amino acid difference (Arg instead of Gly) with the deduced amino acid sequence of the porcine cDNA clone isolated.