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Applicability of SSCP analysis for MHC genotyping: fingerprinting of Ovar‐DRB1 exon 2 alleles from Finnish and Russian breeds
Author(s) -
Kostia S.,
Kantanen J.,
Kolkkala M.,
Varvio SL.
Publication year - 1998
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1998.296376.x
Subject(s) - biology , genotyping , single strand conformation polymorphism , exon , genetics , allele , major histocompatibility complex , polymorphism (computer science) , genotype , gene
The applicability of single strand conformation polymorphism (SSCP) analysis for major histocompatibility complex (MHC) genotyping in sheep was studied. A panel of Ovar‐DRB1 exon 2 ‘allele fingerprints’ was defined. The panel could accelerate DRB1 genotyping of new breeds when already existing sequences are used as references in SSCP analysis. In this study, seven new exon 2 sequences and 19 different alleles in total were detected from 31 animals of Finnish and Russian sheep breeds. Ovar‐DRB1 * 0201 was detected in all the six grey Finnsheep animals included in this study, suggesting reduced MHC diversity within these animals.