Premium
Genomic structure of the bovine PrP gene and complete nucleotide sequence of bovine PrP cDNA
Author(s) -
Horiuchi M.,
Ishiguro N.,
Nagasawa H.,
Toyoda Y.,
Shinagawa M.
Publication year - 1998
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.1998.00250.x
Subject(s) - biology , bovine genome , complementary dna , nucleic acid sequence , genetics , gene , sequence (biology) , single nucleotide polymorphism , microbiology and biotechnology , genome , genotype
The present authors previously reported the nucleotide sequence of the 5′ half of a cDNA encoding bovine prion protein (PrP) and the genomic structure of the bovine PrP gene encoding the 5′‐untranslated region. Here they report the extent of intron 2 of the bovine PrP gene and the nucleotide sequence of the 3′ half of bovine PrP cDNA that had not been determined before. This newly sequenced 3′ half of the bovine PrP cDNA consisted of 2149 bp. The entire 3′‐untranslated region (3′‐UTR) was found to be encoded by a single exon, exon 3. One nucleotide polymorphism was found in the 3′‐UTR. The length of intron 2 was estimated to be about 14 kbp. The structure of bovine PrP gene can be defined by combining the present results and previous reports on the bovine PrP gene.