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A novel in vitro effect of the mucosal protective agent sofalcone – inhibition of chemotactic motility in Helicobacter pylori
Author(s) -
Yoshiyama H.,
Nakamura H.,
Okamoto T.,
Okita K.,
Nakazawa T.
Publication year - 2000
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.2000.014s1230.x
Subject(s) - motility , chemotaxis , chemotaxis assay , microbiology and biotechnology , agar , helicobacter pylori , biology , chocolate agar , bacteria , chemistry , biochemistry , genetics , receptor
Summary Background : Motility of Helicobacter pylori is essential for colonization. H. pylori has been shown to exhibit chemotactic activity toward urea and sodium and bicarbonate ions, which are secreted from the gastric epithelia. The importance of urease activity for chemotactic motility of H. pylori in a viscous environment has also been shown. Consequently, application of drugs inhibiting chemotactic motility has been proposed as a strategy for H. pylori eradication. This inhibitory effect can be evaluated through assay of chemotaxis and swarming. Materials and methods : H. pylori CPY3401 and ATCC43504 were grown on brucella agar plates/broth supplemented with 3% horse serum under microaerobic conditions (N 2 , 85%; O 2 , 5%; CO 2 , 10%). For motility assay, H. pylori cells grown on brucella‐serum agar were stabbed into motility agar containing 0.35% refined agar in brucella‐serum broth and the swarming zone was measured. For the chemotaxis assay, cells were suspended in 10 m m potassium phosphate buffer, pH 7.0, with 3% polyvinyl‐ pyrrolidone and assayed as described previously. Bacterial swimming in the fluid environment was observed under dark‐field microscopy. Results : Numbers of bacteria attracted toward 1 μ m flurofamide were reduced with increasing con‐ centrations of sofalcone (0.2–222 μ m ). In addition, the size of the swarming zone was reduced in motility agar containing 22 and 222 μ m sofalcone. On the other hand, 22 μ m sofalcone did not inhibit bacterial growth on day 3. Bacterial swimming speed in brucella broth was slower in the presence of 22 and 222 μ m sofalcone than in its absence. Conclusion : Sofalcone was found to inhibit chemotactic motility of H. pylori . This drug may be useful for inhibiting the bacterium's ability to colonize the human stomach.