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Matrix metalloproteinase expression in cultured human gastric wall fibroblasts – interactions with Helicobacter pylori isolated from patients with ulcers
Author(s) -
Yokoyama T.,
Otani Y.,
Kurihara N.,
Sakurai Y.,
Kameyama K.,
Suzuki H.,
Igarashi N.,
Kimata M.,
Wada N.,
Kubota T.,
Kumai K.,
Kitajima M.
Publication year - 2000
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.2000.014s1193.x
Subject(s) - helicobacter pylori , matrix metalloproteinase , pathogenesis , extracellular matrix , spirillaceae , medicine , fibroblast , metalloproteinase , in vitro , pathology , immunology , microbiology and biotechnology , biology , gastroenterology , gastritis , biochemistry
Summary Background : Matrix metalloproteinases (MMPs), enzymes capable of degrading collagens and other extracellular matrix components, have been implicated in gastric ulcer formation. However, the effect on MMP expression of Helicobacter pylori , also implicated in these lesions, has not been studied to our knowledge. Aim : To seek links between H. pylori and MMP expression likely to affect gastric ulcer formation. After fibroblasts from human gastric wall were cocultured with H. pylori , concentrations of MMP‐1 and ‐2 in the medium were determined by enzyme‐linked immuno‐sorbent assays. Results : Whereas MMP‐1 was not detected in media from fibroblasts or H. pylori culture alone, MMP‐1 was detected in cocultures ( P < 0.01). Similar amounts of MMP‐2 were detected in medium from fibroblasts cultured alone and with H. pylori . No MMP‐2 production by H. pylori cultured alone was detected. Conclusions : MMP‐1 appears to be important in gastric ulcer pathogenesis, and MMP‐1 induction by H. pylori may impede gastric ulcer healing.