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Nitric oxide formation in the dog sphincter of Oddi from nitric oxide donors as measured with in vivo micro‐dialysis
Author(s) -
Yamamoto I.,
Fujimura M.,
Kihara N.,
Kumano K.,
Yamada T.,
Yamamoto H.,
Fujimiya M.
Publication year - 2000
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.2000.00811.x
Subject(s) - sphincter of oddi , nitric oxide , medicine , sphincter , common bile duct , in vivo , bile duct , polyamine , gastroenterology , endocrinology , anesthesia , chemistry , surgery , biochemistry , biology , microbiology and biotechnology
Background: Nitric oxide (NO) is known to play an important role in neurally mediated relaxation of the sphincter of Oddi. Aim: We investigated whether NO donors, such as nitroglycerin or zwitterionic polyamine/NO, applied into the common bile duct or intravenously, may induce the relaxation of the sphincter of Oddi by producing NO in the anaesthetized dog. Methods: NO production in the sphincter of Oddi was measured by detecting NO oxidation products (NO 2– and NO 3– ) using micro‐dialysis methods. Results: Zwitterionic polyamine/NO and nitroglycerin applied into the common bile duct induced a marked increase in NO 2– but not NO 3– , in the sphincter of Oddi. Intravenous infusion of zwitterionic polyamine/NO and nitroglycerin induced little or no increase in NO 2– formation. Nitroglycerin infused into either the common bile duct or intravenously administered produced relaxation of the sphincter of Oddi, but zwitterionic polyamine/NO had no effect on the sphincter of Oddi in spite of the increase in NO 2– levels. Conclusions: Locally or systemically applied NO donors induce relaxation of the sphincter of Oddi by producing NO, although their mode of action differs in different analogues.