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Chemotactic properties of ICAM‐1 and PECAM‐1 on neutrophil granulocytes in ulcerative colitis: effects of prednisolone and mesalazine
Author(s) -
Vainer B.,
Nielsen O. H.
Publication year - 2000
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.2000.00797.x
Subject(s) - mesalazine , ulcerative colitis , medicine , prednisolone , granulocyte , chemotaxis , immunology , colitis , gastroenterology , receptor , disease
Background: ICAM‐1 seems to exhibit effects other than passive leucocyte/endothelial interaction. Aim: To investigate the attracting properties of selected adhesion molecules, assessing the influence of the two major anti‐inflammatory drugs in ulcerative colitis, prednisolone and mesalazine. Methods: Circulating neutrophils (11 ulcerative colitis, 15 controls) were assessed in microchemotaxis chambers by the leading front technique, using physiologically relevant concentrations of ICAM‐1 (0.005–5000 p M ), PECAM‐1 (0.001–1000 n M ), and P‐selectin (0.01–100 n M ). Neutrophils pre‐incubated with prednisolone (10 –8 –10 –4 M) or mesalazine (0.65–10.4 n M ) were assessed towards ICAM‐1. Results: Migration of neutrophils towards ICAM‐1 showed a bell‐shaped curve with a maximum at 5 p M (migration: 37.7 μm; P < 0.001), whereas PECAM‐1 attracted neutrophils equally in the range of 0.1–10 n M (25.0 μm; P < 0.001). P‐selectin had no cell‐attracting effect. No differences were detected between cells from ulcerative colitis patients and controls. Pre‐treatment with prednisolone decreased the cell attracting effect of ICAM‐1 in a dose‐dependent manner to 72% of the basal migration ( P < 0.001). Conversely, prednisolone showed a pro‐chemokinetic effect by increasing the spontaneous locomotion of neutrophils by 40% ( P < 0.001). Conclusions: Specific chemotactic properties were observed for ICAM‐1 and PECAM‐1. Prednisolone exhibited a dual effect in inhibiting the ICAM‐1‐mediated migration and stimulating the general locomotion of neutrophils.