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Polaprezinc protects gastric mucosal cells from noxious agents through antioxidant properties in vitro
Author(s) -
Hideyuki Hiraishi,
Takako Sasai,
Takeshi Oinuma,
Tadahito Shimada,
Hitoshi Sugaya,
Akira Terano
Publication year - 1999
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.1999.00458.x
Subject(s) - medicine , in vitro , antioxidant , pharmacology , chemistry , biochemistry
Background: Polaprezinc has been shown to exert an anti‐oxidant property in a tube experiment, protect gastric mucosa from experimental ulcerations in vivo , and accelerate the healing of gastric ulcer in humans. Aim: To examine a possible protective effect of polaprezinc on oxidant‐mediated injury in primary monolayer cultures of rat gastric fundic mucosa. Methods: Cytotoxicity was quantified by measuring 51 Cr release. Whether or not polaprezinc exerts an antioxidant property was investigated by determining the effect of this agent on hydrogen peroxide (H 2 O 2 )‐induced injury. The effects of polaprezinc on superoxide (O 2 –· ) generation as well as on ethanol (EtOH)‐induced injury were also examined. Generation of O 2 –· was assessed by the reduction in cytochrome c . Results: H 2 O 2 caused a time‐ and dose‐dependent increase in 51 Cr release. The dose‐response curve of 51 Cr release by H 2 O 2 shifted to the right in the presence of polaprezinc. Polaprezinc, at submillimolar concentrations, prevented H 2 O 2 ‐induced 51 Cr release. EtOH also caused a dose‐dependent increase in 51 Cr release, which was prevented by the addition of polaprezinc. The incubation of cells with EtOH caused an increase in cytochrome c reduction, as the concentrations of EtOH increased. Polaprezinc inhibited EtOH‐induced cytochrome c reduction. Protection by polaprezinc was microscopically associated with the prevention of monolayer disruption. Conclusions: Polaprezinc is antioxidative and directly protects gastric mucosal cells from noxious agents through its antioxidant properties in vitro . This finding may provide the theoretical basis for the usage of an antiulcer drug with antioxidant properties for the treatment of gastric inflammation, such as that induced by ethanol.