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Insulin‐like growth factor I plays a role in gastric wound healing: evidence using a zinc derivative, polaprezinc, and an in vitro rabbit wound repair model
Author(s) -
Sumio Watanabe,
X E Wang,
Miyoko Hirose,
T Kivilioto,
Taro Osada,
Hiroto Miwa,
H. Oide,
Tsuneo Kitamura,
Tomoyuki Yoneta,
Kazuto Seto,
Nobuhiro Sato
Publication year - 1998
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.1998.00408.x
Subject(s) - wound healing , hepatocyte growth factor , fibroblast , growth factor , cancer research , microbiology and biotechnology , medicine , chemistry , biology , in vitro , immunology , biochemistry , receptor
Background: Although the detailed mechanism is unclear, zinc and its derivative, polaprezinc, have been reported to accelerate gastric ulcer healing in vivo . Aim: To investigate the detailed cellular mechanism of polaprezinc on gastric epithelial cells and fibroblasts with special attention to insulin‐like growth factor I (IGF‐I). Methods: Isolated rabbit gastric epithelial cells formed a complete monolayer, from which a circular artificial wound with constant size was made. The restoration process was monitored by measuring wound size up to 48 h. Either polaprezinc, IGF‐I, fibroblast conditioned medium or neutralized medium conditioned by anti‐IGF‐I antibody was added at the time of wounding. The expression of mRNA of IGF‐I, hepatocyte growth factor (HGF) and transforming growth factor α (TGF‐α) in fibroblasts with or without polaprezinc treatment was tested using reverse transcription polymerase chain reaction (RT‐PCR). Gastric epithelial cell proliferation was also examined by bromodeoxyuridine (BrdU) staining. Results: IGF‐I and fibroblast conditioned medium treatment accelerated gastric epithelial restoration which included cell migration and proliferation. However, polaprezinc and neutralized conditioned medium treatment did not accelerate epithelial repair. RT‐PCR for growth factor mRNA revealed the IGF‐I mRNA expression in fibroblasts was increased after treatment with polaprezinc. Conclusion: Polaprezinc induced IGF‐I production from mesenchymal cells, resulting in stimulation of epithelial cell restoration through a paracrine pathway. IGF‐I may play an important role in gastric wound repair.