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Effects of antioxidative agents on apoptosis induced by ischaemia‐reperfusion in rat intestinal mucosa
Author(s) -
Kojima M.,
Iwakiri R.,
Wu B.,
Fujise T.,
Watanabe K.,
Lin T.,
Amemori S.,
Sakata H.,
Shimoda R.,
Oguzu T.,
Ootani A.,
Tsunada S.,
Fujimoto K.
Publication year - 2003
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.18.s1.16.x
Subject(s) - apoptosis , superior mesenteric artery , ischemia , medicine , intestinal mucosa , oxidative stress , reactive oxygen species , pharmacology , reperfusion injury , immunology , pathology , endocrinology , biology , biochemistry
Summary Background & aim : We have previously demonstrated that ischaemia‐reperfusion induces apoptosis in the intestinal mucosa. To evaluate that reactive oxygen species enhanced intestinal apoptosis after ischaemia‐reperfusion, we examined whether antioxidants reduced apoptosis. Methods : Rats were infused through a duodenal tube with antioxidative agents, glutathione, rebamipide and dymethylsulfoxide during 2 h before an ischaemic insult. The superior mesenteric artery was occluded for 60 min, followed by 60 min reperfusion. Apoptosis was evaluated by percentage fragmented DNA (fragmented DNA/total DNA) and immunochemical staining. Results : Increase in apoptosis in the intestinal mucosa after ischaemia‐reperfusion was attenuated by intraduodenal infusion of antioxidative agents, but was not completely abolished. Conclusion : Scavenging effects of the antioxidative agents attenuated increases in intestinal apoptosis, indicating that oxidative stress after ischaemia‐reperfusion plays an important role in induction of apoptosis in the intestinal mucosa.