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Expression of midkine and receptor‐like protein tyrosine phosphatase (RPTP)‐β genes in the rat stomach and the influence of rebamipide
Author(s) -
Yuki T.,
Ishihara S.,
Rumi M.,
OrtegaCava Cesar F.,
Kadowaki Y.,
Kazumori H.,
Yuki M.,
Wada T.,
Miyaoka Y.,
Yoshino N.,
Kinoshita Y.
Publication year - 2003
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1046/j.1365-2036.18.s1.12.x
Subject(s) - midkine , rebamipide , stomach , protein tyrosine phosphatase , cancer research , microbiology and biotechnology , gene expression , western blot , medicine , biology , receptor , gene , growth factor , biochemistry
Summary Background : Midkine has been reported to bind to receptor‐like protein tyrosine phosphatase (RPTP)‐β and to play important roles in growth and differentiation of various cells. Midkine is expressed in rat stomach during experimental ulcer healing, suggesting that the midkine‐RPTP‐β system has some physiological functions in the stomach. Rebamipide is a mucoprotective drug used for the treatment of gastric ulcers. We have tested the hypothesis that the ulcer healing mechanism stimulated by rebamipide is linked physiologically to the gastric midkine‐RPTP‐β system. Materials and methods : Seven‐week‐old‐male Wistar rats were used. Midkine and RPTP‐β gene expression in rat stomach was investigated by laser capture microdissection coupled with the reverse transcription‐polymerase chain reaction (RT‐PCR). The effects of rebamipide on midkine and RPTP‐β expression in rat stomach and the gastric epithelial cell line RGM1 were evaluated by RT‐PCR and Northern blot analyses. Results : Midkine and RPTP‐β expression was detected in the gastric mucosal, submucosal and muscle layers. Rebamipide stimulated both midkine and RPTP‐β expression in rat stomach and RGM1 cells. Conclusion : Rebamipide may protect the gastric mucosa by regulating midkine and RPTP‐β expression.