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Application of synthetic peptides to the diagnosis of neurocysticercosis
Author(s) -
Fleury Agnès,
Beltran Constantino,
Ferrer Elizabeth,
Garate Terésa,
Harrison Leslie J. S.,
Parkhouse R. Michael E.,
Garcia Esperanza,
Fragoso Gladis,
CostaCruz Julia,
Biondi Germano,
Agapejev Svetlana,
Sciutto Edda
Publication year - 2003
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1046/j.1360-2276.2003.01132.x
Subject(s) - neurocysticercosis , taenia solium , cysticercosis , taenia , antigen , neglected tropical diseases , virology , biology , helminthiasis , medicine , immunology , pathology , helminths , disease
Summary We tested the possible diagnostic utility of five Taenia saginata oncosphere‐derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme‐linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6‐2 and Ts45W‐1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.