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Interaction of PRMT1 with BTG/TOB proteins in cell signalling: molecular analysis and functional aspects
Author(s) -
Berthet Cyril,
Guéhenneux Fabienne,
Revol Valérie,
Samarut Christiane,
Lukaszewicz Agnès,
Dehay Colette,
Dumontet Charles,
Magaud JeanPierre,
Rouault JeanPierre
Publication year - 2002
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1356-9597.2001.00497.x
Subject(s) - biology , signalling , computational biology , microbiology and biotechnology , genetics
Background: Several recent reports have connected protein methylation with differentiation. Furthermore, the BTG/TOB proteins have also been implicated in such control. BTG1 and 2 have been shown to interact with PRMT1 (predominant cellular arginine N‐methyltransferase of type I). Results: First, we have studied the interaction between PRMT1 and the proteins of the BTG/TOB family. We show that boxC, a sequence present only in BTG1 and BTG2, is essential for this association. Using boxC peptide, we have investigated the importance of PRMT1/BTG protein association during type I protein methylation reactions. Finally, we show that the addition of boxC fused to penetratin interferes with the neuronal differentiation of PC12 cells and ES cell‐derived neurones. Conclusions: Taken together, these results indicate that PRMT1/BTG proteins could play a key role in the arginine methylation‐mediated signalling pathway as well as in neuronal differentiation.