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Regulator protein that potentially triggers the transcription of the H + ‐ATPase operon in a ruminal bacterium, Streptococcus bovis
Author(s) -
OHKAWARA Sou,
ASANUMA Narito,
HINO Tsuneo
Publication year - 2003
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1046/j.1344-3941.2003.00100.x
Subject(s) - operon , streptococcus bovis , transcription (linguistics) , atpase , biology , footprinting , microbiology and biotechnology , regulator , transcriptional regulation , dna footprinting , messenger rna , in vitro , promoter , transcription factor , gene , biochemistry , gene expression , enzyme , mutant , rumen , linguistics , philosophy , fermentation
In order to seek regulator proteins that are involved in the transcription of the H + ‐ATPase operon ( atp ) in Streptococcus bovis , a transcriptional promoter region of the atp was identified. DNase I footprinting revealed the presence of S. bovis cellular protein that binds to the promoter region. In vitro transcription analysis indicated that the cellular protein triggers atp ‐mRNA synthesis. In vitro atp ‐mRNA synthesis increased six‐fold on changing the culture pH from 7.0 to 4.5, suggesting that the potential regulator protein increases in response to low pH.