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Effect of the strain combination of the donor and recipient on the production efficiency of W‐bearing sperm in mixed‐sex germline chimeric chickens
Author(s) -
KAGAMI Hiroshi,
YASUDA Jun,
TAGAMI Takahiro,
NAITO Mitsuru,
MATSUBARA Yuko,
HARUMI Takashi,
NOGUCHI Takamasa,
YAMAMOTO Yasuhiro,
TAKAHASHI Taikai,
MATSUYAMA Junichi,
KOMATSU Hiroyuki,
ONO Tamao
Publication year - 2002
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1046/j.1344-3941.2002.00062.x
Subject(s) - sperm , biology , germline , andrology , chimera (genetics) , spermatogenesis , genetics , endocrinology , medicine , gene
Effect of the strain combination of the donor and recipient on production efficiency of W‐bearing sperm in mixed‐sex chimeric testes was analyzed. The combinations of the donors and recipients were White Leghorn (WL) and Rhode Island Red (RIR), and vice versa. Generated mixed‐sex chimeras that had the male phenotype at sexual maturity were classified into four groups: (1) a female WL donor and a male RIR recipient; (2) a male WL donor and a female RIR recipient; (3) a female RIR donor and a male WL recipient; (4) a male RIR donor and a female WL recipient. The mean number of W‐bearing sperm detected by in situ hybridization among 10 000 sperm observed were 147, 165, 30 and 45 in groups 1, 2, 3 and 4, respectively. The numbers in groups 1 and 2 were both significantly higher than those of groups 3 and 4 ( P < 0.05). The combination of a WL donor and a RIR recipient produced W‐bearing sperm more efficiently than the reverse combination.