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Characterization of ammonia‐assimilating bacteria in a lagoon for wastewater from a paddock of dairy cattle
Author(s) -
SASAKI Hiraku,
MARUYAMA Gen,
SUZUKI Hanatsu,
AKA Jun,
SATO Masaaki,
SASAKI Takako,
OHTA Minoru,
NAKAI Yutaka
Publication year - 2002
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1046/j.1344-3941.2002.00004.x
Subject(s) - microorganism , ammonia , bacteria , wastewater , pseudomonas , nitrogen , food science , biology , chemistry , 16s ribosomal rna , microbiology and biotechnology , environmental chemistry , environmental science , environmental engineering , biochemistry , genetics , organic chemistry
We investigated microorganisms that assimilated ammonia in lagoon treatment processes. Ammonia‐assimilating microorganisms were detected by nitrogen‐limited medium that contained ammonia as the sole nitrogen source. Numbers of ammonia‐assimilating aerobes (log CFU/g) were 3.4, 4.8, 5.0, 4.8 and 5.0 (log CFU/mL) on the culture plate incubated at 4°C, 10°C, 15°C, 20°C and 25°C, respectively. Many isolates used ammonia in high rates when they were purely cultivated in nitrogen‐limited medium added to sterilized lagoon extract. Many of them used ammonia even when they were cultivated in media containing viable microbial flora of the lagoon. Among them, enterobacteriaceae and Pseudomonas sp. were identified by analysis of 16S ribosomal DNA.