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Analysis of aneuploidy in mini‐Percoll gradient centrifuged human sperm for intracytoplasmic sperm injection (ICSI) using fluorescence in situ hybridization
Author(s) -
Kunathikom Somboon,
Rattanachaiyat Manee,
Makemaharn Orawan
Publication year - 2002
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1046/j.1341-8076.2002.00038.x
Subject(s) - intracytoplasmic sperm injection , percoll , sperm , medicine , andrology , aneuploidy , gynecology , chromatography , embryo , biology , chemistry , in vitro fertilisation , microbiology and biotechnology , genetics , centrifugation , gene , chromosome
Aim: To study the aneuploidy rates of chromosomes 13, 18, 21, X and Y in Percoll gradient centrifuged sperm from infertile patients with male infertility factor treated by intracytoplasmic sperm injection (ICSI) compared with healthy fertile donors and infertile patients with normal semen parameters.Methods: This case‐controlled study was conducted in a university hospital. Semen samples were obtained from three healthy fertile donors, eight infertile patients with normal semen parameters, and 18 infertile patients with male infertility factor. All samples were subjected to mini‐Percoll gradient centrifugation before being processed through fluorescent in situ hybridization. The incidences of aneuploidy were compared using Chi‐squared test.Results and Conclusions: A total of 64 949 spermatozoa were analyzed. The disomy rates for chromosomes 13, 18, 21, and X or Y of sperm from patients with male infertility factor were 0.21%, 0.37%, 0.36% and 0.63%, respectively, whereas the diploidy rate was 0.17–0.23%. These incidences were higher than those from men with normal semen parameters. The result suggested that the embryos of patients with male infertility factor treated by ICSI are at increased risk of chromosome abnormalities.